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KMID : 1007520020110020140
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Food Science and Biotechnology
2002 Volume.11 No. 2 p.140 ~ p.146
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Cytotoxicity of Phenolic Compounds Isolated from Seeds of Safflower(Carthamus tinctorius L.) on Cancer Cell Lines
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Bae, Song Ja
Shim, Sun Mi/Park, Yun Ja/Lee, Jun Young/Chang, Eun Ju/Choi, Sang won
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Abstract
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The methanolic extract of roasted safflower seeds exhibited moderate cytotoxicity against three cancer cell lines, HepG2, MCF-7, and Hela, in a dose-dependent manner, as measured by MTT assay. The methanolic extract was further partitioned with n-hexane, ethyl acetate and n-butanol successively. The ethyl acetate fraction exhibited potent cytotoxicities against the cancer cell lines. NFeruloylserotonin (Sl), N-(p-coumaroyl)serotonin (S2), matairesinol (L1), 8´-hydroxyarctigenin (L2), luteolin (Fl), and acacetin (F2) were isolated from the ethyl acetate extract, and their chemical structures were identified by UV, IR, NMR, and MS spectroscopic methods. Six phenolic compounds possessed comparable cytotoxicity against three cancer cells. In particular, Fl and F2 had the most potent cytotoxicity with IC_50 values of 51.8 and 62.1㎍/mL for Hela cell, 33.6 and 37.7㎍/mL for MCF-7 cell and 47.3 and 56.6 .μg/mL for HepG2 cell, respectively. In addition, Ll, L2, Sl, and S2 also showed strong cytotoxicity, although activities of serotonins were weaker than those of lignans. On normal human liver cell (WRL68) at a high concentration of 100 ㎍/mL, two serotonins and lignans did not show any cytotoxicity, and two flavonoids exhibited only about 50% cytotoxicity. These results suggest that phenolic compounds in the safflower seeds may be useful as potential cancer chemopreventive agents.
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